Serrano A, Villalba J M, González-Reyes J A, Navas P, Córdoba F
Departamento de Biología Celular, Universidad de Córdoba, Spain.
Biochem Mol Biol Int. 1994 Apr;32(5):841-9.
Plasma membranes purified by two-phase partition from onion roots catalyzed the NAD(P)H-dependent reduction of a variety of electron acceptor such as ferricyanide, quinones, dyes and ascorbate free radical. Among these, NAD(P)H-ferricyanide and -quinone oxidoreductase activities were effectively solubilized by Triton X-100. Both oxidoreductase activities were bound to an affinity column of Blue-Sepharose CL 6B. NADH eluted a redox enzyme showing more juglone than ferricyanide-dependent activity. Ulterior unspecific elution with salt allowed us to the partial purification of a different redox enzyme of about 31 kDa that reduced better ferricyanide than quinones and constituted the bulk of solubilized redox activity.
通过两相分配从洋葱根中纯化的质膜催化了多种电子受体（如铁氰化物、醌类、染料和抗坏血酸自由基）的NAD(P)H依赖性还原反应。其中，NAD(P)H-铁氰化物和-醌氧化还原酶活性可被Triton X-100有效溶解。两种氧化还原酶活性均与Blue-Sepharose CL 6B亲和柱结合。NADH洗脱了一种氧化还原酶，该酶对胡桃醌的活性比对铁氰化物依赖性活性更强。随后用盐进行非特异性洗脱，使我们能够部分纯化一种约31 kDa的不同氧化还原酶，该酶还原铁氰化物的能力比醌类更好，并且构成了溶解的氧化还原活性的主要部分。
