Revathi C J, Chattopadhyay A, Srinivas U K
Centre for Cellular & Molecular Biology, Hyderabad, India.
Biochem Mol Biol Int. 1994 Apr;32(5):941-50.
In vivo change in membrane organization in adult rat liver cells induced by heat shock was studied by incorporating the fluorescent probes 1,6-diphenyl-1,3,5-hexatriene (DPH) and 1-[4-(trimethylammonio)phenyl]-6-phenyl-1,3,5-hexatriene (TMA-DPH) in these cells. DPH was essentially localized in the plasma membrane, as indicated by quenching of its fluorescence (over 90%) by 2,4,6-trinitrobenzenesulfonate (TNBS). Measurements of both fluorescence polarization and intensity indicate that there is an immediate change in membrane organization (order) following heat shock. The polarization changes of DPH and TMA-DPH incorporated into liver cell plasma membranes upon heat shock are in opposite directions. This result is attributed to the different locations of these probes in the membrane. This change in membrane organization could be a part of a general stress response which is then transmitted to other cellular organelles.
通过将荧光探针1,6 - 二苯基 - 1,3,5 - 己三烯（DPH）和1 - [4 - （三甲基铵基）苯基] - 6 - 苯基 - 1,3,5 - 己三烯（TMA - DPH）掺入成年大鼠肝细胞中，研究了热休克诱导的肝细胞中膜组织的体内变化。DPH主要定位于质膜，这通过2,4,6 - 三硝基苯磺酸盐（TNBS）对其荧光的猝灭（超过90%）得以表明。荧光偏振和强度的测量均表明热休克后膜组织（有序性）立即发生变化。热休克时掺入肝细胞质膜的DPH和TMA - DPH的偏振变化方向相反。该结果归因于这些探针在膜中的不同位置。膜组织的这种变化可能是一般应激反应的一部分，随后传递至其他细胞器。
