Nishiyama N, Cho S I, Kitagawa I, Saito H
Department of Chemical Pharmacology, Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
Biol Pharm Bull. 1994 Apr;17(4):509-13. doi: 10.1248/bpb.17.509.
Effects of malonylginsenoside Rb1 (GRb1-m) isolated from dried root of Panax ginseng C. A. Meyer (Araliaceae) on neuronal survival and neurite outgrowth were compared with those of ginsenoside Rb1 (GRb1) using organ culture of chick embryonic dorsal root ganglia (DRG) and cell culture of DRG neurons. In the organ culture, nerve growth factor (NGF) showed neurite outgrowth promoting effect. GRb1-m (30 microM) significantly potentiated this NGF-induced neurite outgrowth with similar potency to that of GRb1 (30 microM). Low density cell culture of DRG neurons was employed to minimize the contribution of contaminating non-neuronal cells. NGF (10 ng/ml) prolonged duration of neuronal survival. Neither GRb1-m nor GRb1 (1-30 microM) changed the prolongation effect of NGF, nor did NGF show a significant effect on the neurite elongation and re-elongation after axotomy by laser beam irradiation. However, GRb1-m (10 microM) potentiated initial neurite elongation when co-treated with NGF. In the process of re-elongation of neurites, GRb1-m (1, 30 microM) also promoted it in the presence of NGF. These results suggest, first, that GRb1-m potentiates NGF-induced neurite outgrowth of chick embryonic DRGs and DRG neurons, but behaves in a slightly different manner from GRb1, and, second, that the effects of the two saponins may work primarily on neurons causing the potentiation of NGF-induced neurite outgrowth.
利用鸡胚背根神经节（DRG）器官培养和DRG神经元细胞培养，比较了从五加科植物人参（Panax ginseng C. A. Meyer）干燥根中分离得到的丙二酰人参皂苷Rb1（GRb1-m）与人参皂苷Rb1（GRb1）对神经元存活和神经突生长的影响。在器官培养中，神经生长因子（NGF）显示出促进神经突生长的作用。GRb1-m（30 microM）显著增强了这种NGF诱导的神经突生长，其效力与GRb1（30 microM）相似。采用DRG神经元低密度细胞培养以尽量减少污染的非神经元细胞的影响。NGF（10 ng/ml）延长了神经元存活时间。GRb1-m和GRb1（1 - 30 microM）均未改变NGF的延长作用，NGF对激光束照射切断轴突后的神经突伸长和再伸长也无显著影响。然而，GRb1-m（10 microM）与NGF共同处理时增强了初始神经突伸长。在神经突再伸长过程中，GRb1-m（1、30 microM）在有NGF存在时也促进了再伸长。这些结果表明，首先，GRb1-m增强了NGF诱导的鸡胚DRG和DRG神经元的神经突生长，但行为方式与GRb1略有不同；其次，这两种皂苷的作用可能主要作用于神经元，导致NGF诱导的神经突生长增强。
