Emmer A, Jansson M, Roeraade J
Department of Analytical Chemistry, Royal Institute of Technology, Stockholm, Sweden.
J Chromatogr A. 1994 Jun 24;672(1-2):231-6. doi: 10.1016/0021-9673(94)80611-X.
An attempt was made to separate the isoenzymes and subunits of pig liver esterase by capillary zone electrophoresis. This enzyme is a complex mixture and is strongly adsorbed on a fused-silica capillary. However, by simply adding a cationic fluorosurfactant to the running buffer, adsorption was significantly reduced. The effects of adding a zwitterionic and a neutral fluorosurfactant were also investigated. Large changes in the elution pattern were observed when using different combinations of these additives. Mixtures of different fluorosurfactants added to the running buffer can therefore be utilized in strategies for optimization of the separation selectivity.
尝试通过毛细管区带电泳分离猪肝酯酶的同工酶和亚基。这种酶是一种复杂的混合物，会强烈吸附在熔融石英毛细管上。然而，只需在运行缓冲液中添加一种阳离子氟表面活性剂，吸附作用就会显著降低。还研究了添加两性离子和中性氟表面活性剂的效果。当使用这些添加剂的不同组合时，观察到洗脱模式有很大变化。因此，添加到运行缓冲液中的不同氟表面活性剂混合物可用于优化分离选择性的策略中。
