Ovesen T, Gaihede M, Scousboe P, Ledet T
Ear- Nose and Throat Department, Aarhus University Hospital, Denmark.
In Vitro Cell Dev Biol Anim. 1994 Apr;30A(4):249-55. doi: 10.1007/BF02632047.
The present study was undertaken to quantitate the effects of atmospheric air and normal middle ear gas on cultured fibroblasts obtained from normal rabbit middle ear mucosa. The cells were exposed to three different gas compositions: 7% O2:5% CO2:88% N2, 21% O2:5% CO2:74% N2, and 75% O2:5% CO2:20% N2. The growth was monitored by measuring the total content of cell protein, the amount of DNA, and the cell division activity. The activity of the synthetic apparatus was determined by the collagen synthesis. For comparison, rabbit skin fibroblasts were grown under identical conditions. The results demonstrated significantly higher replication rate of middle ear fibroblasts at 7% oxygen than at atmospheric air whereas the collagen synthesis was significantly lower at 7%. Furthermore, the responses varied significantly between rabbit middle ear and rabbit skin fibroblasts. Thus the present study substantiates the hypothesis of an influence of atmospheric air on the middle ear mucosa which might be of importance, e.g., in relation to insertion of ventilation tubes or longstanding perforations of the tympanic membrane in otitis media.
本研究旨在定量分析大气空气和正常中耳气体对从正常兔中耳黏膜获取的培养成纤维细胞的影响。将细胞暴露于三种不同的气体成分中：7%氧气：5%二氧化碳：88%氮气、21%氧气：5%二氧化碳：74%氮气、以及75%氧气：5%二氧化碳：20%氮气。通过测量细胞蛋白质的总含量、DNA的量以及细胞分裂活性来监测细胞生长。合成装置的活性通过胶原蛋白合成来确定。作为对照，兔皮肤成纤维细胞在相同条件下培养。结果表明，与大气空气相比，7%氧气条件下中耳成纤维细胞的复制率显著更高，而7%氧气条件下胶原蛋白合成显著更低。此外，兔中耳成纤维细胞和兔皮肤成纤维细胞的反应存在显著差异。因此，本研究证实了大气空气对中耳黏膜有影响这一假说，这可能具有重要意义，例如，与中耳通气管的插入或中耳炎鼓膜长期穿孔有关。
