Maruniak J E, Garcia-Canedo A, Rodrigues J J
Entomology and Nematology Department, University of Florida, Gainesville 32611.
In Vitro Cell Dev Biol Anim. 1994 Apr;30A(4):283-6. doi: 10.1007/BF02632053.
Four insect cell lines were used to isolate two recombinant baculoviruses which had the beta-galactosidase (beta-gal) gene for colorimetric assay purposes. Plaque assays were performed using two Trichoplusia ni cell lines: BTI-TN-5B1-4 and TN-368, and two Spodptera frugiperda cell lines: IPLB-SF-21AE and SF9. The number of plaques (occlusion positive and blue beta-gal+ recombinants) formed in the Trichoplusia cells was higher than in the Spodoptera cells. The appearance of Autographa californica NPV polyhedra was also faster in the T. ni cell lines. The effect of cell passage on the plaque formation proved to be critical when two different passages of the SF9 cells were tested. The higher passage produced a lower viral titration. The size and time of appearance of the plaques was also different.
使用四种昆虫细胞系来分离两种重组杆状病毒，这两种病毒带有用于比色测定的β-半乳糖苷酶（β-gal）基因。使用两种粉纹夜蛾细胞系：BTI-TN-5B1-4和TN-368，以及两种草地贪夜蛾细胞系：IPLB-SF-21AE和SF9进行噬斑测定。在粉纹夜蛾细胞中形成的噬斑（包涵体阳性和蓝色β-gal+重组体）数量高于草地贪夜蛾细胞。苜蓿银纹夜蛾核型多角体病毒多角体在粉纹夜蛾细胞系中的出现也更快。当测试SF9细胞的两种不同传代时，细胞传代对噬斑形成的影响被证明至关重要。传代次数越高，病毒滴度越低。噬斑的大小和出现时间也不同。
