Ultrastructural localization of endogenous hormones and receptors in bone tissue: an immunocytological approach in frozen samples.

In addition to their well-known mechanical role, bones are essential for the regulation of calcium phosphate metabolism. Most of the peptide and steroid hormones acting on bone tissue exert their cellular effects directly on bone cells after binding to cell receptors. A combined method using immunocytology on ultrathin frozen sections has been applied to bone tissue and made possible the ultrastructural localization of endogenous hormones and hormone receptors. There is immunocytological evidence, at the osteoblast level, for: (1) the presence of calcitonin, parathyroid hormone, growth hormone, 1,25 dihydroxyvitamin D3, aldosterone, corticosterone and progesterone; (2) the existence of 1,25 dihydroxyvitamin D3 and progesterone receptors; (3) the absence of estradiol and receptors, testosterone and gonadoliberin. Ultimate goals of future studies are to extend the ultrastructural localization of hormones at the osteoclast level, to assess the distribution of adhesive proteins and of their receptors in bone cells and matrix, and to use well-calcified human bone samples in order to evaluate better the role of therapeutic agents.