Daugaard H, Egfjord M, Lewin E, Olgaard K
Medical Department P, Rigshospitalet, Copenhagen, Denmark.
Exp Nephrol. 1994 Jul-Aug;2(4):240-8.
The metabolism of synthetic human intact PTH (1-84), 1,000 or 3 pmol/l, was studied in isolated perfused livers and in filtering and nonfiltering kidneys from normal rats and rats made chronically uremic by 5/6 nephrectomy. Clearances were measured by assays specific for intact PTH, and NH2-terminal, mid-molecule, and COOH-terminal iPTH. Release of PTH fragments was analyzed by HPLC. Clearance of the added intact PTH by the uremic kidneys was reduced by the same order of magnitude as GFR. Neither the uremic nor the normal kidneys released any iPTH fragments. No peritubular metabolism of intact PTH was found in the uremic nonfiltering kidneys. Clearance of intact PTH by uremic and control livers was not significantly different. The uremic and control livers released equal amounts of COOH-terminal iPTH fragments, but no NH2-terminal fragments. Thus, uremia per se did not influence the renal and hepatic metabolism of PTH.
在正常大鼠以及通过5/6肾切除造成慢性尿毒症的大鼠的离体灌注肝脏、滤过和非滤过肾脏中，研究了1000或3 pmol/l合成人完整甲状旁腺激素（1-84）的代谢情况。通过针对完整甲状旁腺激素、氨基末端、中分子和羧基末端甲状旁腺激素相关肽（iPTH）的特异性测定来测量清除率。通过高效液相色谱法分析甲状旁腺激素片段的释放情况。尿毒症肾脏对添加的完整甲状旁腺激素的清除率与肾小球滤过率（GFR）以相同数量级降低。尿毒症肾脏和正常肾脏均未释放任何iPTH片段。在尿毒症非滤过肾脏中未发现完整甲状旁腺激素的肾小管周围代谢。尿毒症肝脏和对照肝脏对完整甲状旁腺激素的清除率无显著差异。尿毒症肝脏和对照肝脏释放等量的羧基末端iPTH片段，但未释放氨基末端片段。因此，尿毒症本身并不影响甲状旁腺激素的肾脏和肝脏代谢。
