Blitzer R D, Wong T, Nouranifar R, Landau E M
Department of Psychiatry, Mount Sinai Medical Center, New York, NY 10029.
Brain Res. 1994 May 23;646(2):312-4. doi: 10.1016/0006-8993(94)90096-5.
The possible involvement of protein kinase A (PKA) in the muscarinic inhibition of the slow afterhyperpolarizing current (IAHP) was investigated in rat hippocampal pyramidal cells. IAHP was recorded using the whole cell method in hippocampal slices, and Rp-cAMPS, a PKA antagonist, was applied intracellularly. The inhibition of IAHP by carbachol was not affected by Rp-cAMPS. In contrast, Rp-cAMPS reduced the cAMP-dependent inhibition of IAHP by norepinephrine. The results show that phosphorylation by PKA does not contribute to the muscarinic effect on IAHP.
在大鼠海马锥体细胞中研究了蛋白激酶A（PKA）可能参与毒蕈碱对缓慢超极化后电流（IAHP）的抑制作用。采用全细胞膜片钳方法在海马脑片中记录IAHP，并向细胞内施加PKA拮抗剂Rp-cAMPS。卡巴胆碱对IAHP的抑制作用不受Rp-cAMPS的影响。相反，Rp-cAMPS减弱了去甲肾上腺素对IAHP的cAMP依赖性抑制作用。结果表明，PKA介导的磷酸化作用不参与毒蕈碱对IAHP的作用。
