大鼠海马中复极化后胆碱能抑制作用独立于环磷酸腺苷依赖性蛋白激酶。
The cholinergic inhibition of afterhyperpolarization in rat hippocampus is independent of cAMP-dependent protein kinase.
作者信息
Blitzer R D, Wong T, Nouranifar R, Landau E M
机构信息
Department of Psychiatry, Mount Sinai Medical Center, New York, NY 10029.
出版信息
Brain Res. 1994 May 23;646(2):312-4. doi: 10.1016/0006-8993(94)90096-5.
The possible involvement of protein kinase A (PKA) in the muscarinic inhibition of the slow afterhyperpolarizing current (IAHP) was investigated in rat hippocampal pyramidal cells. IAHP was recorded using the whole cell method in hippocampal slices, and Rp-cAMPS, a PKA antagonist, was applied intracellularly. The inhibition of IAHP by carbachol was not affected by Rp-cAMPS. In contrast, Rp-cAMPS reduced the cAMP-dependent inhibition of IAHP by norepinephrine. The results show that phosphorylation by PKA does not contribute to the muscarinic effect on IAHP.
在大鼠海马锥体细胞中研究了蛋白激酶A（PKA）可能参与毒蕈碱对缓慢超极化后电流（IAHP）的抑制作用。采用全细胞膜片钳方法在海马脑片中记录IAHP，并向细胞内施加PKA拮抗剂Rp-cAMPS。卡巴胆碱对IAHP的抑制作用不受Rp-cAMPS的影响。相反，Rp-cAMPS减弱了去甲肾上腺素对IAHP的cAMP依赖性抑制作用。结果表明，PKA介导的磷酸化作用不参与毒蕈碱对IAHP的作用。
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