阿片类物质对人神经母细胞瘤细胞系SK-N-SH中3H-去甲肾上腺素释放的双重调节作用

Dual regulation by opioids of 3H-norepinephrine release in the human neuroblastoma cell-line SK-N-SH.

作者信息

Keren O, Garty M, Sarne Y

机构信息

Clinical Pharmacology Unit-Medicine F, Beilinson Medical Center, Petah-Tiqva, Israel.

出版信息

Brain Res. 1994 May 23;646(2):319-23. doi: 10.1016/0006-8993(94)90098-1.

DOI:10.1016/0006-8993(94)90098-1

PMID:8069682

Abstract

Depolarization-evoked 3H-norepinephrine release from SK-N-SH cells was found to be regulated by opioid ligands. Opioids exerted either inhibition or augmentation of 3H-norepinephrine release. Both effects were mediated by opioid receptors. In addition, a nonopioid inhibitory effect of opiates on release was observed. The SK-N-SH cell-line provides a suitable model for studying the various mechanisms underlying the opioid regulatory pathways within single cells.

摘要

研究发现，去极化诱发的SK-N-SH细胞释放3H-去甲肾上腺素受阿片样物质配体调控。阿片样物质对3H-去甲肾上腺素的释放可产生抑制或增强作用。这两种作用均由阿片受体介导。此外，还观察到阿片类药物对释放有非阿片样抑制作用。SK-N-SH细胞系为研究单细胞内阿片样物质调节途径的各种机制提供了一个合适的模型。