Weiger R, Friedrich C, Netuschil L, Schlagenhauf U
Dental School of Medicine, Department of Conservative Dentistry, University of Tübingen, FRG.
Caries Res. 1994;28(4):267-71. doi: 10.1159/000261984.
The effect on local plaque formation of a varnish containing 1% chlorhexidine and 1% thymol (Cervitec) was evaluated. Ten volunteers with clinically healthy oral conditions were asked to refrain from any kind of oral hygiene measures for three periods of three days. Undisturbed plaque formation was recorded during the first experimental period. At the beginning of the second period, the varnish was applied to six vestibular enamel surfaces and removed after 1 h. The third experimental period was initiated 12 weeks after varnish application to assess a potential long-term effect. During each period of plaque formation, samples were collected from the vestibular surfaces after 24 h (from teeth 15/25), after 48 h (from teeth 14/24) and after 72 h (from teeth 13/23), respectively, and evaluated for total microscopic bacterial counts (BC) and colony forming units (CFU). Microbial vitality was assessed by the plating efficiency [PE = (CFU/BC) x 100] and directly by a vital fluorescence (VF) technique. VF of 48- and 72-hour plaque was significantly reduced after Cervitec application. An inhibitory effect by Cervitec could not be discerned 12 weeks after varnish treatment.
评估了含1%氯己定和1%百里酚的清漆（Cervitec）对局部菌斑形成的影响。10名口腔临床健康的志愿者被要求在三个为期三天的时间段内避免采取任何口腔卫生措施。在第一个实验期记录未受干扰的菌斑形成情况。在第二个阶段开始时，将该清漆涂覆在六个前庭釉质表面，并在1小时后去除。在涂覆清漆12周后开始第三个实验期，以评估潜在的长期效果。在每个菌斑形成期，分别在24小时（从15/25号牙）、48小时（从14/24号牙）和72小时（从13/23号牙）后从前庭表面采集样本，并评估总显微镜下细菌计数（BC）和菌落形成单位（CFU）。通过接种效率[PE =（CFU/BC）×100]并直接通过活菌荧光（VF）技术评估微生物活力。应用Cervitec后，48小时和72小时菌斑的VF显著降低。在清漆治疗12周后未发现Cervitec有抑制作用。
