Yan S Q, Wang G J, Shen T Y
Department of Orthopaedics, University of Virginia, Charlottesville.
Clin Orthop Relat Res. 1994 Sep(306):239-46.
Typha angustata Bony et Chaub. is a traditional Chinese medicine, commonly used in China for a variety of clinical disorders, including atherosclerosis, cardiovascular diseases, uterus contraction, and wound healing. The effect of the pollen of Typha angustata on the bone inductive capacity of demineralized bone matrix is studied here. Demineralized bone matrix soaked with saline solution was implanted in 8-mm defects in rat calvaria. After surgery all rats received a 0.2-ml injection in the defect sites of Typha angustata extract, plasma, or saline 3 times weekly for 2 to 4 weeks. The repair of bone defects was evaluated by radiography and by histology at 2 and 4 weeks after surgery. Results indicated that the 3% Typha angustata extract and demineralized bone matrix combination produced substantially more bone than demineralized bone matrix alone, while plasma plus demineralized bone matrix induced the same amount of bone formation as Typha angustata extract plus demineralized bone matrix. The osteoinductive potential increased in a dose dependent manner, 3% Typha angustata extract plus demineralized bone matrix produced more bone than the 0.6% Typha angustata extract plus demineralized bone matrix at 2 and 4 weeks. This study demonstrates that an extract of the pollen of Typha angustata is capable of enhancing the osteoinductive potential of demineralized bone matrix.
狭叶香蒲是一种传统中药，在中国常用于治疗多种临床疾病，包括动脉粥样硬化、心血管疾病、子宫收缩和伤口愈合。本文研究了狭叶香蒲花粉对脱矿骨基质骨诱导能力的影响。将用盐溶液浸泡过的脱矿骨基质植入大鼠颅骨的8毫米缺损处。术后所有大鼠每周3次在缺损部位注射0.2毫升狭叶香蒲提取物、血浆或生理盐水，持续2至4周。在术后2周和4周通过放射学和组织学评估骨缺损的修复情况。结果表明，3%狭叶香蒲提取物与脱矿骨基质组合产生的骨量明显多于单独的脱矿骨基质，而血浆加脱矿骨基质诱导的骨形成量与狭叶香蒲提取物加脱矿骨基质相同。骨诱导潜力呈剂量依赖性增加，在2周和4周时，3%狭叶香蒲提取物加脱矿骨基质产生的骨量多于0.6%狭叶香蒲提取物加脱矿骨基质。本研究表明，狭叶香蒲花粉提取物能够增强脱矿骨基质的骨诱导潜力。
