编码黄顶菊碳酸酐酶的互补DNA的核苷酸序列。
The nucleotide sequence of a complementary DNA encoding Flaveria bidentis carbonic anhydrase.
作者信息
Cavallaro A, Ludwig M, Burnell J
机构信息
Centre for Molecular Biotechnology, Queensland University of Technology, Brisbane, Australia.
出版信息
FEBS Lett. 1994 Aug 22;350(2-3):216-8. doi: 10.1016/0014-5793(94)00767-5.
We have isolated and characterised a cDNA clone encoding the cytosolic form of carbonic anhydrase in the leaves of Flaveria bidentis, a C4 dicotyledonous plant. The deduced amino acid sequence is similar to the carbonic anhydrase found in the chloroplasts of C3 dicotyledonous plants. Western blot analysis of crude leaf extracts of F. bidentis indicates that the leader sequence (equivalent to the transit peptide of the chloroplastic form of CA found in C3 plants) is not removed following translation of mRNA.
我们已经从C4双子叶植物二齿叶黄菊（Flaveria bidentis）的叶片中分离并鉴定了一个编码碳酸酐酶胞质形式的cDNA克隆。推导的氨基酸序列与在C3双子叶植物叶绿体中发现的碳酸酐酶相似。对二齿叶黄菊粗叶提取物的蛋白质免疫印迹分析表明，mRNA翻译后前导序列（相当于C3植物中发现的叶绿体形式CA的转运肽）未被去除。
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