Engels P, Fichtel K, Lübbert H
Preclinical Research, Sandoz Pharma Ltd., Basel, Switzerland.
FEBS Lett. 1994 Aug 22;350(2-3):291-5. doi: 10.1016/0014-5793(94)00788-8.
Type IV phosphodiesterases (PDE IV) specifically hydrolyze cAMP and are inhibited by rolipram. RT-PCR was applied to analyze the expression patterns of mRNAs for four cloned human and rat phosphodiesterase type IV isogenes (PDE IV-A, -B, -C and -D). Although these patterns were mostly coincident for the human and rat PDE IV genes, some differences were found between the two species. PDE IV-A expression was detectable in human blood but not in rat blood, suggesting a species-specific difference in the expression of this PDE IV isogene. PDE IV-C was neither detected in human or rat blood nor in different cell populations of the human immune system. It is further demonstrated that the PDE IV isogene expression is differentially regulated by cAMP in different cell types.
IV型磷酸二酯酶（PDE IV）特异性水解环磷酸腺苷（cAMP），并被咯利普兰抑制。采用逆转录聚合酶链反应（RT-PCR）分析4种克隆的人及大鼠IV型磷酸二酯酶基因（PDE IV-A、-B、-C和-D）的mRNA表达模式。尽管人和大鼠PDE IV基因的这些模式大多一致，但在这两个物种之间发现了一些差异。PDE IV-A在人血液中可检测到，但在大鼠血液中未检测到，提示该PDE IV基因在表达上存在种属特异性差异。在人血液或大鼠血液以及人免疫系统的不同细胞群体中均未检测到PDE IV-C。进一步证明，PDE IV基因在不同细胞类型中受cAMP的差异调节。
