Takahashi M, Furukawa T, Tanaka I, Ohsawa Y, Nikkuni K, Oaki A, Goto T, Hashimoto T, Kishi K, Koike T
First Department of Internal Medicine, Niigata University, School of Medicine, Japan.
Hematol Oncol. 1994 Mar-Apr;12(2):53-60. doi: 10.1002/hon.2900120202.
In order to clarify the function of P210 bcr/abl oncogene in leukemogenesis, IL-3 dependent murine hematopietic cell line, FDC-P2, was transfected with the plasmid containing cDNA of P210 bcr/abl oncogene (pGD'210) or murine IL-3 (pcDmIL3) by electroporation. Four out of five pGDH210 transfected clones as well as FDC-P2 transfected with pcDmIL3, acquired autonomous proliferation (i.e. lost the requirement for IL-3 supplementation). The expression of bcr/abl oncogene was weak in one clone, which remained dependent on IL-3. Unlike pcDmIL3 transfectants, which secrete IL-3 into the supernatant, IL-3 was not demonstrated in the culture supernatant of pGD'210 transfected FDC-P2. These finding suggest that P210 bcr/abl oncogene is directly associated with autonomous proliferation, which is the first process of leukemogenesis.
为阐明P210 bcr/abl癌基因在白血病发生中的作用，通过电穿孔法将含有P210 bcr/abl癌基因cDNA的质粒（pGD'210）或小鼠白细胞介素-3（pcDmIL3）转染至依赖白细胞介素-3的小鼠造血细胞系FDC-P2中。五个转染pGDH210的克隆中有四个以及转染pcDmIL3的FDC-P2获得了自主增殖能力（即不再需要补充白细胞介素-3）。有一个克隆中bcr/abl癌基因的表达较弱，该克隆仍依赖白细胞介素-3。与向培养上清液中分泌白细胞介素-3的pcDmIL3转染子不同，在转染pGD'210的FDC-P2的培养上清液中未检测到白细胞介素-3。这些发现表明，P210 bcr/abl癌基因与自主增殖直接相关，而自主增殖是白血病发生的第一步。
