Tan M, Gou Q, Hou Y, Wu M
Hua Xi Yi Ke Da Xue Xue Bao. 1994 Mar;25(1):105-7.
ORM1 is an important genetic marker for both parentage testing and personal identification in forensic science. We have examined the ORM1 phenotypes in the human serum and dried bloodstains using the ULPAGIF method. ORM1 phenotypes were successfully demonstrated from dried bloodstains stored at 4 degrees C and room temperature for up to twenty-four weeks. In bloodstains stored at 37 degrees C, the 100% of ORM1 phenotyping was no longer possible after 12 weeks. We examined 9 bloodstains stored at room temperature for one, two and three years, respectively. For the one-year-old bloodstains, all of the samples could be phenotyped correctly, for the two-year-old bloodstains 8 out of the 9 and for the three-year-old bloodstains 7 out of the 9 samples could be phenotyped correctly. The results have indicated that ORM1 is relatively stable, for it is tolerable toward high temperature and long duration. It was suggested that the bloodstains should be ultrasonicated before ULPAGIF, because the ultrasonication could elevate the detectable rate of ORM1 phenotypes from bloodstains. ORM1 phenotypes was performed in five disputed parentage cases. One of the alleged fathers was excluded.
ORM1是法医学中用于亲子鉴定和个人识别的重要遗传标记。我们使用超低压聚丙烯酰胺凝胶免疫固定电泳（ULPAGIF）方法检测了人血清和干血斑中的ORM1表型。在4℃和室温下储存长达24周的干血斑中成功检测到了ORM1表型。在37℃储存的血斑中，12周后就不再可能100%进行ORM1表型分析。我们分别检测了在室温下储存1年、2年和3年的9个血斑。对于1年的血斑，所有样本都能正确进行表型分析；对于2年的血斑，9个样本中有8个；对于3年的血斑，9个样本中有7个能正确进行表型分析。结果表明，ORM1相对稳定，因为它能耐受高温和长时间。建议在进行ULPAGIF之前对血斑进行超声处理，因为超声处理可以提高血斑中ORM1表型的检测率。对5起有争议的亲子鉴定案件进行了ORM1表型分析。排除了一名被指控的父亲。
