Lin P, Xiao Y, Cao J, Zhang J, Lu Y, Huang X
Hua Xi Yi Ke Da Xue Xue Bao. 1994 Mar;25(1):35-7.
Molecular weight of human hepatocellular carcinoma (HCC)-associated antigen-HL2 antigen was detected to be about 50,000 dalton by SDS-PAGE and Western blot. One hundred and twenty-seven paraffin sections of cancers and pathological liver tissue were tests by ABC immunohistochemical staining. MAb HL2 reacted with 62.7% HCC and also with 8 of the 10 stomach carcinomas but only with a few other digestive system carcinomas (pancreas carcinoma, rectum carcinoma and duodenum carcinoma) and hepatitis. MAb HL2 was negative in hepatocirrhosis and other tumors (vesica carcinoma, skin carcinoma, breast carcinoma, neurogliocytoma and carcinoma of the lung). The results suggest that MAb HL2 has values in diagnosis of HCC and differential diagnosis between HCC and other tumours. HL2 antigen might be a new tumor-associated antigen (TAA). Further study of HL2 antigen will significantly show the actions of TAA in the occurrence and development of tumor.
通过SDS-PAGE和蛋白质印迹法检测发现，人肝细胞癌（HCC）相关抗原-HL2抗原的分子量约为50,000道尔顿。采用ABC免疫组织化学染色法对127例癌症石蜡切片和病理肝脏组织进行检测。单克隆抗体HL2与62.7%的肝癌发生反应，也与10例胃癌中的8例发生反应，但仅与少数其他消化系统癌症（胰腺癌、直肠癌和十二指肠癌）以及肝炎发生反应。单克隆抗体HL2在肝硬化和其他肿瘤（膀胱癌、皮肤癌、乳腺癌、神经胶质瘤和肺癌）中呈阴性。结果表明，单克隆抗体HL2在肝癌诊断以及肝癌与其他肿瘤的鉴别诊断中具有价值。HL2抗原可能是一种新的肿瘤相关抗原（TAA）。对HL2抗原的进一步研究将显著揭示TAA在肿瘤发生和发展中的作用。
