Monoclonal antibody raised against apoprotein of C-1027: effect on biochemical and biological activities of the holoantibiotic.

A monoclonal antibody raised against C-1027 apoprotein recognized not only the apoprotein, but also the holoantibiotic (Antibiotic C-1027) almost equally. Among the biochemical and biological activities of the holoantibiotic, the antibody inhibited the aminopeptidase activity and the cytotoxicity to Ehrlich carcinoma cells in cultures, but not the DNA-cleaving activity in vitro. The immunohistogram, using this antibody, of Ehrlich carcinoma cells that had been exposed to the holoantibiotic suggested penetration of the holoantibiotic into target cells.