Margolin Y, Barak R, Eisenbach M
Department of Membrane Research and Biophysics, Weizmann Institute of Science, Rehovot, Israel.
J Bacteriol. 1994 Sep;176(17):5547-9. doi: 10.1128/jb.176.17.5547-5549.1994.
The effect of arsenate on flagellar rotation in cytoplasm-free flagellated envelopes of Escherichia coli and Salmonella typhimurium was investigated. Flagellar rotation ceased as soon as the envelopes were exposed to arsenate. Inclusion of phosphate intracellularly (but not extracellular) prevented the inhibition by arsenate. In a parallel experiment, the rotation was not affected by inclusion of an ATP trap (hexokinase and glucose) within the envelopes. It is concluded that arsenate affects the motor in a way other than reversible deenergization. This may be an irreversible damage to the cell or direct inhibition of the motor by arsenate. The latter possibility suggests that a process of phosphorylation or phosphate binding is involved in the motor function.
研究了砷酸盐对大肠杆菌和鼠伤寒沙门氏菌无细胞质鞭毛包膜中鞭毛旋转的影响。一旦包膜暴露于砷酸盐，鞭毛旋转就会停止。细胞内（而非细胞外）加入磷酸盐可防止砷酸盐的抑制作用。在一项平行实验中，包膜内加入ATP阱（己糖激酶和葡萄糖）对旋转没有影响。得出的结论是，砷酸盐对马达的影响方式并非可逆去能。这可能是对细胞的不可逆损伤或砷酸盐对马达的直接抑制。后一种可能性表明，磷酸化或磷酸盐结合过程参与了马达功能。
