细胞色素P450 2C3在兔肝微粒体中对1-硝基芘和3-硝基荧蒽的代谢作用。
The role of cytochrome P450 2C3 in rabbit liver microsomal metabolism of 1-nitropyrene and 3-nitrofluoranthene.
作者信息
Howard P C, McManus M E, Koop D R
机构信息
Department of Environmental Health Sciences, School of Medicine, Case Western Reserve University, Cleveland, OH 44106-4940.
出版信息
J Biochem Toxicol. 1994 Apr;9(2):71-8. doi: 10.1002/jbt.2570090204.
The male rabbit liver microsomal cytochrome P450 metabolism of 1-nitropyrene and 3-nitrofluoranthene was investigated. In this study, we used inhibitory antibodies specific for rabbit P450 2C3 and determined that, in untreated male rabbit liver microsomes, the antibody inhibited approximately 75% of the cytochrome P450-mediated C-oxidation of both 1-nitropyrene and 3-nitrofluoranthene. These results verify our previous prediction that mainly one cytochrome P450 is responsible for microsomal metabolism of 1-nitropyrene in the untreated rabbit liver.
研究了雄性兔肝微粒体细胞色素P450对1-硝基芘和3-硝基荧蒽的代谢。在本研究中，我们使用了对兔P450 2C3具有特异性的抑制性抗体，并确定在未处理的雄性兔肝微粒体中，该抗体抑制了细胞色素P450介导的1-硝基芘和3-硝基荧蒽C-氧化反应的约75%。这些结果证实了我们之前的预测，即在未处理的兔肝中，主要有一种细胞色素P450负责微粒体对1-硝基芘的代谢。
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