Thompson R E, Reynolds H Y, Waxdal M J
Biochemistry. 1975 Jul;14(13):2853-60. doi: 10.1021/bi00684a010.
Dog serum and colostral immunoglobulin A (IgA) and free secretory component from colostrum were isolated using affinity chromatography. Both serum and colostral IgA showed similar susceptibility to reduction with dithiothreitol, but only colostral IgA released the additional subunit, bound secretory component. This released secretory component was identical with free secretory component with respect to electrophoretic migration, isoelectric focusing point, and molecular weight, but lacked some antigenic determinants. The amino acid composition and the N-terminal sequence of canine free secretory component was similar to that reported for the cow.
使用亲和色谱法分离犬血清、初乳免疫球蛋白A（IgA）和初乳中的游离分泌成分。血清和初乳IgA对二硫苏糖醇还原的敏感性相似，但只有初乳IgA释放出额外的亚基，即结合的分泌成分。这种释放出的分泌成分在电泳迁移率、等电聚焦点和分子量方面与游离分泌成分相同，但缺乏一些抗原决定簇。犬游离分泌成分的氨基酸组成和N端序列与报道的牛的相似。
