Liao Z X, Travis E L
Department of Experimental Radiotherapy, University of Texas M. D. Anderson Cancer Center, Houston 77030.
Radiat Res. 1994 Sep;139(3):290-9.
The effect of unilateral nephrectomy 24 h after irradiation on renal function and death with renal insufficiency as well as histopathological changes in the kidney was assessed. Single doses totaling 8-18 Gy were given bilaterally to unanesthetized female and male C3Hf/Kam mice. Renal function damage was assayed by blood urea nitrogen (BUN) and hematocrit.(Hct). Histological damage was quantified by two parameters: kidney area and number of surviving tubule cells along the renal capsule. The number of glomeruli was scored as an indication of the number of nephrons. Changes in the two functional parameters did not appear sooner after irradiation in the nephrectomized mice than in the non-nephrectomized mice. Rather, less impairment of function was measured by both parameters in the nephrectomized mice but only after radiation doses greater than 12 Gy. The LD50 at 424 days after irradiation was also higher in the nephrectomized mice than that in the mice receiving only irradiation, 13.98 Gy (95% confidence limits = 12.03, 15.93) and 11.71 Gy (95% confidence limits = 10.4, 13.1), respectively, in agreement with the data on function. Unilateral nephrectomy alone induced a 10% increase in size of the contralateral kidney. The dose-response curve for the kidney area from nephrectomized mice was parallel to and displaced above that for non-nephrectomized mice, indicating that the increase in renal mass occurred independent of and was not compromised by radiation. Unilateral nephrectomy alone induced no increase in the number of proximal tubules in the contralateral kidney. However, tubule survival was higher in nephrectomized mice given doses greater than 12 Gy compared with mice receiving only radiation. Fitting the tubule survival data by maximum likelihood analysis gave D0's of 6.7 Gv (95% confidence limits = 6.3, 7.1 Gy) and 3.7 Gy (95% confidence limits = 3.5, 3.8 Gy) for the irradiated nephrectomized mice and irradiated mice, respectively. However, the number of glomeruli was the same for both groups, suggesting that the number of the nephrons did not change. These data suggest that the improvement in renal function in mice nephrectomized 24 h after irradiation of both kidneys compared to those receiving only irradiation was due to tubule hyperplasia and not renal hypertrophy.(ABSTRACT TRUNCATED AT 400 WORDS)
评估了照射后24小时单侧肾切除对肾功能、死于肾功能不全以及肾脏组织病理学变化的影响。对未麻醉的雌性和雄性C3Hf/Kam小鼠双侧给予总量为8 - 18 Gy的单次剂量照射。通过血尿素氮（BUN）和血细胞比容（Hct）测定肾功能损害。组织学损害通过两个参数进行量化：肾脏面积和沿肾被膜存活的肾小管细胞数量。肾小球数量作为肾单位数量的指标进行评分。在切除肾脏的小鼠中，照射后这两个功能参数的变化并不比未切除肾脏的小鼠出现得更早。相反，在切除肾脏的小鼠中，仅在辐射剂量大于12 Gy后，这两个参数测量的功能损害较小。照射后424天，切除肾脏的小鼠的半数致死剂量（LD50）也高于仅接受照射的小鼠，分别为13.98 Gy（95%置信区间 = 12.03, 15.93）和11.71 Gy（95%置信区间 = 10.4, 13.1），这与功能数据一致。单独单侧肾切除导致对侧肾脏大小增加10%。切除肾脏的小鼠肾脏面积的剂量 - 反应曲线与未切除肾脏的小鼠平行且位于其上方，表明肾脏质量的增加独立于辐射且未受辐射影响。单独单侧肾切除未导致对侧肾脏近端小管数量增加。然而，与仅接受辐射的小鼠相比，给予大于12 Gy剂量的切除肾脏的小鼠肾小管存活率更高。通过最大似然分析拟合肾小管存活数据，照射的切除肾脏的小鼠和照射的小鼠的D0分别为6.7 Gv（95%置信区间 = 6.3, 7.1 Gy）和3.7 Gy（95%置信区间 = 3.5, 3.8 Gy）。然而，两组的肾小球数量相同，表明肾单位数量没有变化。这些数据表明，与仅接受照射的小鼠相比，双侧肾脏照射后24小时切除肾脏的小鼠肾功能的改善是由于肾小管增生而非肾肥大。（摘要截断于400字）
