Taccioli G E, Gottlieb T M, Blunt T, Priestley A, Demengeot J, Mizuta R, Lehmann A R, Alt F W, Jackson S P, Jeggo P A
Howard Hughes Medical Institute, Children's Hospital, Boston, MA.
Science. 1994 Sep 2;265(5177):1442-5. doi: 10.1126/science.8073286.
The radiosensitive mutant xrs-6, derived from Chinese hamster ovary cells, is defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. The human XRCC5 DNA repair gene, which complements this mutant, is shown here through genetic and biochemical evidence to be the 80-kilodalton subunit of the Ku protein. Ku binds to free double-stranded DNA ends and is the DNA-binding component of the DNA-dependent protein kinase. Thus, the Ku protein is involved in DNA repair and in V(D)J recombination, and these results may also indicate a role for the Ku-DNA-dependent protein kinase complex in those same processes.
源自中国仓鼠卵巢细胞的放射敏感突变体xrs-6在DNA双链断裂修复和进行V(D)J重组的能力方面存在缺陷。通过遗传和生化证据表明，可弥补该突变体的人类XRCC5 DNA修复基因是Ku蛋白的80千道尔顿亚基。Ku与游离的双链DNA末端结合，是DNA依赖性蛋白激酶的DNA结合成分。因此，Ku蛋白参与DNA修复和V(D)J重组，这些结果也可能表明Ku-DNA依赖性蛋白激酶复合物在相同过程中发挥作用。
