Greenfeld J I, Noizat-Pirenne F, Hardy M A, Oluwole S F, Hesdorffer C A, Preti R A, De Groote D, Franchimont P
Department of Surgery, Columbia University College of Physicians and Surgeons, New York, New York 10032.
Transplantation. 1994 Aug 27;58(4):504-10. doi: 10.1097/00007890-199408270-00018.
To determine the degree of graft versus host disease (GVHD) prophylaxis that might be necessary if cord blood (CB) transplantation is more widely applied, we compared human cord blood mononuclear cell (CBMC) and adult peripheral blood mononuclear cell (PBMC) proliferative responses and stimulatory capabilities; to examine the utility of UVB irradiation for GVHD prophylaxis, we compared proliferative responses, antigen-presenting cell (APC) stimulatory functions, and cytokine production by untreated and UVB-irradiated CBMCs. The two cell types, CBMC and PBMC, proliferated equally both in response to phytohemagglutinin (PHA) and alloantigen in mixed lymphocyte culture (MLC). Cord blood stimulatory function in MLC was significantly (P < 0.05) reduced to 60% of PBMC stimulatory capability. Ultraviolet-B irradiation at a dose of 100 J/m2 of CBMCs significantly (P < 0.01) inhibited PHA stimulation by 79.4%, reduced responder activity in MLC by 75.8%, and inhibited stimulatory activity in MLC by 55.6% as compared with the activity shown by untreated CBMCs. The same dose of UVB preserved 59.9% of CFU-GM and 65.9% of BFU-E colony growth as compared with untreated CBMCs. Production of lymphokines (IL-2, GM-CSF, LIF, and gamma-IFN) by PHA-stimulated CBMCs was decreased, but monokine (IL-1 beta and IL-6) production was unchanged. We conclude that UVB irradiation at a dose of 100 J/m2 inhibits CB lymphocyte activation and preserves the cellular growth potential of CB hematopoietic progenitor cells.
为了确定如果脐血（CB）移植得到更广泛应用时可能所需的移植物抗宿主病（GVHD）预防程度，我们比较了人脐血单个核细胞（CBMC）和成人外周血单个核细胞（PBMC）的增殖反应及刺激能力；为了研究紫外线B（UVB）照射用于预防GVHD的效用，我们比较了未处理的和经UVB照射的CBMC的增殖反应、抗原呈递细胞（APC）刺激功能及细胞因子产生情况。在混合淋巴细胞培养（MLC）中，CBMC和PBMC这两种细胞类型对植物血凝素（PHA）和同种异体抗原的增殖反应相同。MLC中脐血的刺激功能显著（P<0.05）降低至PBMC刺激能力的60%。与未处理的CBMC相比，100 J/m2剂量的UVB照射显著（P<0.01）抑制PHA刺激达79.4%，使MLC中的反应活性降低75.8%，并使MLC中的刺激活性降低55.6%。与未处理的CBMC相比，相同剂量的UVB可保留59.9%的集落形成单位-粒细胞-巨噬细胞（CFU-GM）和65.9%的爆式红系集落形成单位（BFU-E）集落生长。PHA刺激的CBMC产生的淋巴细胞因子（白细胞介素-2、粒细胞-巨噬细胞集落刺激因子、白血病抑制因子和γ-干扰素）减少，但单核因子（白细胞介素-1β和白细胞介素-6）产生未变。我们得出结论，100 J/m2剂量的UVB照射可抑制CB淋巴细胞活化，并保留CB造血祖细胞的细胞生长潜能。
