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[腺病毒5在穿透阶段于HeLa细胞质内迁移的免疫荧光研究]

[Immunofluorescence study of the migration of adenovirus 5 in HeLa cell cytoplasm during the penetration phase].

作者信息

Lyon M, Chardonnet Y

出版信息

C R Acad Hebd Seances Acad Sci D. 1975 Apr 28;280(16):1919-22.

PMID:807382
Abstract

Using the indirect immunofluorescence technique in presence of anti-adenovirus-5 antibody, fluorescent spots were observed two hours after adsorption at 4 degrees C onto HeLa cells on the surface of the cells. The spots subsequently became intracytoplasmic and persisted about 7 to 8 h. Intense fluorescence was further observed in the whole cytoplasm and in the nucleus 12 h following the injection.

摘要

在抗腺病毒-5抗体存在的情况下,使用间接免疫荧光技术,在4℃吸附到HeLa细胞表面两小时后观察到荧光斑点。这些斑点随后进入细胞质并持续约7至8小时。注射后12小时,在整个细胞质和细胞核中进一步观察到强烈荧光。

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