Desoize B, Broglio C, Gorisse M C, Briois F, Carpentier Y, Delvincourt C, Dufer J
GIBSA, Institut Jean Godinot, Reims, France.
Anticancer Res. 1994 May-Jun;14(3A):995-1000.
Resistance of Friend murine erythroleukemia cells was induced or selected by continuous stepwise exposure to adriamycin (ADM). The resistance index (R.I.) varied with different "mdr type" drugs, even when the compounds were closely related as ADM and daunorubicin (DNR). The cell uptake of anthracycline, evaluated by flow cytometry (FCM), was better correlated with the R.I. than the HPLC assessment. The quantitative cytology showed nuclear changes (size and color distribution); all the modifications were in part dependent on the degree of resistance. This study showed that the graded resistant sublines differed in their resistant phenotypes apart from their different degree of resistance.
通过连续逐步暴露于阿霉素（ADM）诱导或筛选出Friend小鼠红白血病细胞的耐药性。即使这些化合物如ADM和柔红霉素（DNR）密切相关，耐药指数（R.I.）也会因不同的“mdr类型”药物而有所不同。通过流式细胞术（FCM）评估的蒽环类药物的细胞摄取与耐药指数的相关性比高效液相色谱（HPLC）评估更好。定量细胞学显示细胞核有变化（大小和颜色分布）；所有这些改变部分取决于耐药程度。这项研究表明，除了耐药程度不同外，分级耐药亚系在耐药表型上也存在差异。
