Hollender J, Dott W, Hopp J
Fachgebiet Hygiene, Technische Universität Berlin, Germany.
Appl Environ Microbiol. 1994 Jul;60(7):2330-8. doi: 10.1128/aem.60.7.2330-2338.1994.
Comamonas testosteroni JH5 was isolated from a mixed bacterial culture enriched on different chloro- and methylphenols. The strain completely mineralized a mixture consisting of 4-chlorophenol (4-CP) and 4-methylphenol (4-MP). During degradation of the mixture, 4-hydroxybenzyl alcohol, 4-hydroxybenzaldehyde, 4-hydroxybenzoic acid, and 4-chlorocatechol were detected as short-lived intermediates. Mineralization of 4-CP and that of 4-MP occurred successively and were accompanied by diauxic growth, whereas 4-CP and 2-methylphenol were mineralized simultaneously. It was ascertained that neither a reversible enzyme inhibition nor potential toxic intermediates caused the observed diauxie. Some facts support the hypothesis that the successive degradation of 4-CP and 4-MP is regulated on the level of transcription. C. testosteroni JH5 contained a meta-cleaving enzyme when pregrown on 4-CP and the isomeric monomethylphenols. Inactivation of this enzyme in the presence of 3-chlorocatechol was observed.
睾丸酮丛毛单胞菌JH5是从富集了不同氯酚和甲基酚的混合细菌培养物中分离得到的。该菌株能完全矿化由4-氯酚（4-CP）和4-甲基酚（4-MP）组成的混合物。在混合物降解过程中，检测到4-羟基苄醇、4-羟基苯甲醛、4-羟基苯甲酸和4-氯儿茶酚为短暂存在的中间产物。4-CP和4-MP的矿化相继发生，并伴随着二次生长，而4-CP和2-甲基酚则同时被矿化。已确定，观察到的二次生长既不是由可逆酶抑制也不是由潜在的有毒中间产物引起的。一些事实支持这样的假说，即4-CP和4-MP的相继降解是在转录水平上受到调控的。睾丸酮丛毛单胞菌JH5在以4-CP和异构单甲基酚预培养时含有一种间位裂解酶。在3-氯儿茶酚存在的情况下观察到了该酶的失活。
