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从耐热链霉菌中克隆编码3 - O - 酰基转移酶的大环内酯类抗生素生物合成基因acyA及其在直接发酵生产杂合大环内酯类抗生素中的应用。

Cloning of the macrolide antibiotic biosynthesis gene acyA, which encodes 3-O-acyltransferase, from Streptomyces thermotolerans and its use for direct fermentative production of a hybrid macrolide antibiotic.

作者信息

Arisawa A, Kawamura N, Takeda K, Tsunekawa H, Okamura K, Okamoto R

机构信息

Mercian Corporation, Central Research Laboratories, Kanagawa, Japan.

出版信息

Appl Environ Microbiol. 1994 Jul;60(7):2657-60. doi: 10.1128/aem.60.7.2657-2660.1994.

DOI:10.1128/aem.60.7.2657-2660.1994
PMID:8074537
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC201700/
Abstract

A gene encoding the macrolide modification enzyme 3-O-acyltransferase (acyA) was cloned by chromosome walking onto the carbomycin biosynthetic region in Streptomyces thermotolerans TH475, with the 3' region of the gene encoding the macrolide modification enzyme 4"-O-acyltransferase (acyB1) as a probe. A shortened fragment (1.8 kb) containing acyA was subcloned with pIJ350. A high-level tylosin producer, Streptomyces fradiae MBBF, transformed with the plasmid could produce a hybrid macrolide, 3-O-acetyltylosin, most efficiently.

摘要

以编码大环内酯修饰酶4″-O-酰基转移酶（acyB1）的基因3′区域为探针，通过染色体步移法克隆了编码大环内酯修饰酶3-O-酰基转移酶（acyA）的基因，该基因位于耐热链霉菌TH475的碳霉素生物合成区域。将含有acyA的缩短片段（1.8 kb）亚克隆到pIJ350中。用该质粒转化的高产泰乐菌素的弗氏链霉菌MBBF能够最有效地产生一种杂合大环内酯3-O-乙酰泰乐菌素。

相似文献

1
Cloning of the macrolide antibiotic biosynthesis gene acyA, which encodes 3-O-acyltransferase, from Streptomyces thermotolerans and its use for direct fermentative production of a hybrid macrolide antibiotic.从耐热链霉菌中克隆编码3 - O - 酰基转移酶的大环内酯类抗生素生物合成基因acyA及其在直接发酵生产杂合大环内酯类抗生素中的应用。
Appl Environ Microbiol. 1994 Jul;60(7):2657-60. doi: 10.1128/aem.60.7.2657-2660.1994.
2
Direct fermentative production of acyltylosins by genetically-engineered strains of Streptomyces fradiae.通过弗氏链霉菌基因工程菌株直接发酵生产酰基泰乐菌素。
J Antibiot (Tokyo). 1996 Apr;49(4):349-54. doi: 10.7164/antibiotics.49.349.
3
Nucleotide sequence analysis of the carbomycin biosynthetic genes including the 3-O-acyltransferase gene from Streptomyces thermotolerans.耐热链霉菌中包括3-O-酰基转移酶基因在内的碳霉素生物合成基因的核苷酸序列分析。
Biosci Biotechnol Biochem. 1995 Apr;59(4):582-8. doi: 10.1271/bbb.59.582.
4
Cloning and nucleotide sequences of two genes involved in the 4''-O-acylation of macrolide antibiotics from Streptomyces thermotolerans.
Biosci Biotechnol Biochem. 1993 Dec;57(12):2020-5. doi: 10.1271/bbb.57.2020.
5
Production of a hybrid macrolide antibiotic in Streptomyces ambofaciens and Streptomyces lividans by introduction of a cloned carbomycin biosynthetic gene from Streptomyces thermotolerans.通过导入来自耐热链霉菌的克隆碳霉素生物合成基因，在浅青紫链霉菌和变铅青链霉菌中生产杂合大环内酯类抗生素。
Gene. 1989 Dec 28;85(2):293-301. doi: 10.1016/0378-1119(89)90421-6.
6
The mycarose-biosynthetic genes of Streptomyces fradiae, producer of tylosin.泰乐菌素产生菌弗氏链霉菌的霉糖生物合成基因。
Microbiology (Reading). 2000 Jan;146 ( Pt 1):139-146. doi: 10.1099/00221287-146-1-139.
7
Characterization and targeted disruption of a glycosyltransferase gene in the tylosin producer, Streptomyces fradiae.泰乐菌素产生菌弗氏链霉菌中一种糖基转移酶基因的表征与靶向破坏
Gene. 1998 Jul 3;214(1-2):95-100. doi: 10.1016/s0378-1119(98)00210-8.
8
Molecular analysis of tlrB, an antibiotic-resistance gene from tylosin-producing Streptomyces fradiae, and discovery of a novel resistance mechanism.来自泰乐菌素产生菌弗氏链霉菌的抗生素抗性基因tlrB的分子分析及一种新抗性机制的发现。
J Antibiot (Tokyo). 1999 Mar;52(3):288-96. doi: 10.7164/antibiotics.52.288.
9
Molecular analysis of tlrD, an MLS resistance determinant from the tylosin producer, Streptomyces fradiae.对来自泰乐菌素产生菌弗氏链霉菌的MLS耐药决定子tlrD的分子分析。
Gene. 1996 Nov 21;180(1-2):173-6. doi: 10.1016/s0378-1119(96)00448-9.
10
[Deletion of spiramycin 3-O-acyltransferase gene from Streptomyces spiramyceticus F21 resulting in the production of spiramycin I as major component].[从螺旋链霉菌F21中缺失螺旋霉素3-O-酰基转移酶基因导致以螺旋霉素I作为主要成分的生产]
Sheng Wu Gong Cheng Xue Bao. 2007 Jul;23(4):612-7.

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Molecular characterization of the oafA locus responsible for acetylation of Salmonella typhimurium O-antigen: oafA is a member of a family of integral membrane trans-acylases.负责鼠伤寒沙门氏菌O抗原乙酰化的oafA基因座的分子特征：oafA是完整膜转酰基酶家族的一员。
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本文引用的文献

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Cloning and nucleotide sequences of two genes involved in the 4''-O-acylation of macrolide antibiotics from Streptomyces thermotolerans.
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Production of a hybrid macrolide antibiotic in Streptomyces ambofaciens and Streptomyces lividans by introduction of a cloned carbomycin biosynthetic gene from Streptomyces thermotolerans.通过导入来自耐热链霉菌的克隆碳霉素生物合成基因，在浅青紫链霉菌和变铅青链霉菌中生产杂合大环内酯类抗生素。
Gene. 1989 Dec 28;85(2):293-301. doi: 10.1016/0378-1119(89)90421-6.
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