从耐热链霉菌中克隆编码3 - O - 酰基转移酶的大环内酯类抗生素生物合成基因acyA及其在直接发酵生产杂合大环内酯类抗生素中的应用。
Cloning of the macrolide antibiotic biosynthesis gene acyA, which encodes 3-O-acyltransferase, from Streptomyces thermotolerans and its use for direct fermentative production of a hybrid macrolide antibiotic.
作者信息
Arisawa A, Kawamura N, Takeda K, Tsunekawa H, Okamura K, Okamoto R
机构信息
Mercian Corporation, Central Research Laboratories, Kanagawa, Japan.
出版信息
Appl Environ Microbiol. 1994 Jul;60(7):2657-60. doi: 10.1128/aem.60.7.2657-2660.1994.
Abstract
A gene encoding the macrolide modification enzyme 3-O-acyltransferase (acyA) was cloned by chromosome walking onto the carbomycin biosynthetic region in Streptomyces thermotolerans TH475, with the 3' region of the gene encoding the macrolide modification enzyme 4"-O-acyltransferase (acyB1) as a probe. A shortened fragment (1.8 kb) containing acyA was subcloned with pIJ350. A high-level tylosin producer, Streptomyces fradiae MBBF, transformed with the plasmid could produce a hybrid macrolide, 3-O-acetyltylosin, most efficiently.
摘要
以编码大环内酯修饰酶4″-O-酰基转移酶（acyB1）的基因3′区域为探针，通过染色体步移法克隆了编码大环内酯修饰酶3-O-酰基转移酶（acyA）的基因，该基因位于耐热链霉菌TH475的碳霉素生物合成区域。将含有acyA的缩短片段（1.8 kb）亚克隆到pIJ350中。用该质粒转化的高产泰乐菌素的弗氏链霉菌MBBF能够最有效地产生一种杂合大环内酯3-O-乙酰泰乐菌素。
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Cloning of the macrolide antibiotic biosynthesis gene acyA, which encodes 3-O-acyltransferase, from Streptomyces thermotolerans and its use for direct fermentative production of a hybrid macrolide antibiotic.从耐热链霉菌中克隆编码3 - O - 酰基转移酶的大环内酯类抗生素生物合成基因acyA及其在直接发酵生产杂合大环内酯类抗生素中的应用。
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