DeVault J D, Narang S K
United States Department of Agriculture, Agricultural Research Service, Fargo, ND 58105.
Biochem Biophys Res Commun. 1994 Aug 30;203(1):169-75. doi: 10.1006/bbrc.1994.2164.
In the present study, a PCR-based approach was undertaken to determine if members of the hobo-like family of transposable elements were identifiable within the genomes of a number of diverse species, including Heliothis virescens and Helicoverpa zea. The amplified products derived from both H. virescens and H. zea were cloned and characterized. Analysis of the DNA sequence and the single open reading frame found within these fragments clearly demonstrates that these elements are closely related to the hobo transposon from Drosophila melanogaster. In addition, amino acid sequence analysis of the members of this family defines consensus, specific amino acids found within similar regions of all members of this transposon family.
在本研究中,采用了基于聚合酶链式反应(PCR)的方法,以确定在多种不同物种的基因组中是否能够识别出类hobo转座元件家族的成员,这些物种包括烟草天蛾和玉米螟。从烟草天蛾和玉米螟中获得的扩增产物被克隆并进行了特征分析。对这些片段内的DNA序列和单个开放阅读框的分析清楚地表明,这些元件与黑腹果蝇的hobo转座子密切相关。此外,对该家族成员的氨基酸序列分析确定了该转座子家族所有成员相似区域内共有的、特定的氨基酸。