Luque E A, Lemanski L F, Dube D K
Department of Anatomy and Cell Biology, SUNY Health Science Center at Syracuse 13210.
Biochem Biophys Res Commun. 1994 Aug 30;203(1):319-25. doi: 10.1006/bbrc.1994.2184.
The cDNA for alpha-tropomyosin (TM) was cloned by the polymerase chain reaction (PCR) from a lambda gt11 library constructed with mRNA from juvenile axolotl heart tissues. Subsequently, the nucleotide sequence of the cDNA was determined. This is the first reported cDNA for axolotl alpha-tropomyosin. Comparative analyses of the deduced amino acid sequence of this cDNA with Xenopus skeletal muscle alpha-tropomyosin sequences indicate that the axolotl heart cDNA has 93% and 96% homology in the regions of amino acids 39-80 (exon 2b) and 258-284 (exon 9a), respectively. However, there is about 86.55% homology at the nucleic acid level (coding region) and 97.5% homology at the amino acid level with that of Xenopus skeletal muscle alpha-tropomyosin cDNA. Northern blot analyses with polyA+ RNA from juvenile heart suggest the presence of at least two different transcripts for alpha-tropomyosin in axolotl heart. The results of 3'-RACE concur with those of northern blot analyses.
通过聚合酶链反应（PCR）从用幼年美西螈心脏组织的mRNA构建的λgt11文库中克隆了α-原肌球蛋白（TM）的cDNA。随后，测定了该cDNA的核苷酸序列。这是首次报道的美西螈α-原肌球蛋白的cDNA。对该cDNA推导的氨基酸序列与非洲爪蟾骨骼肌α-原肌球蛋白序列进行比较分析表明，美西螈心脏cDNA在氨基酸39 - 80（外显子2b）和258 - 284（外显子9a）区域分别具有93%和96%的同源性。然而，在核酸水平（编码区）与非洲爪蟾骨骼肌α-原肌球蛋白cDNA约有86.55%的同源性，在氨基酸水平有97.5%的同源性。用幼年心脏的polyA + RNA进行的Northern印迹分析表明，美西螈心脏中存在至少两种不同的α-原肌球蛋白转录本。3'-RACE的结果与Northern印迹分析的结果一致。
