一种使用甲状旁腺激素N端的大鼠血清甲状旁腺激素放射免疫测定法。
A radioimmunoassay for rat serum parathyroid hormone using N-terminal of PTH.
作者信息
Fujita T, Okano K, Ohata M, Yoshikawa M
出版信息
Endocrinol Jpn. 1975 Apr;22(2):159-62. doi: 10.1507/endocrj1954.22.159.
Partially purified bovine parathyroid hormone (PTH) produced two kinds of antisera, GP-100 reacting with 125I-labeled bovine PTH (b-PTH 1-84) alone and GP-M reacting with 125I-labeled synthetic N-terminal portion of bovine PTH (b-PTH 1-34) as well as b-PTH 1-84. Rat serum PTH reacted with the latter but not in the former, suggesting a similarity to b-PTH 1-34. Rat serum PTH became undetectable after parathyroidectomy, and increased after Na2-EDTA injection and following renal injury with sodium sulfacetylthiazole.
部分纯化的牛甲状旁腺激素（PTH）产生了两种抗血清，GP - 100仅与125I标记的牛PTH（b - PTH 1 - 84）反应，而GP - M与125I标记的牛PTH合成N端部分（b - PTH 1 - 34）以及b - PTH 1 - 84反应。大鼠血清PTH与后者反应，但不与前者反应，这表明其与b - PTH 1 - 34相似。甲状旁腺切除术后大鼠血清PTH检测不到，而在注射Na2 - EDTA后以及用磺胺乙酰噻唑损伤肾脏后升高。
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