Pancer Z, Gershon H, Rinkevich B
Israel Oceanographic and Limnological Research, National Institute of Oceanography, Dept. of Marine Biology, Haifa.
Biochem Biophys Res Commun. 1994 Aug 30;203(1):646-51. doi: 10.1006/bbrc.1994.2231.
Five microsatellite loci of the marine protochordate Botryllus schlosseri were cloned: four of uninterrupted (AG)n repeats and one of both (AG)n and (TG)n repeats. By means of an innovative procedure small colony fragments were minimally treated to serve as templates for PCR with microsatellite-specific primers. Four of the loci were polymorphic: 7-8 discrete alleles were scored in nine colonies, heterozygosity ranging between 44-80%. At locus number 811 spacing of the alleles and gel-resolution were highest, therefore, ten additional colonies were typed and in total nine alleles were scored with maximal allelic interval of 120 base pair and 53% heterozygous colonies. The high levels of microsatellite-polymorphism provide a new tool as individual markers for studies on aspects of the botryllid polymorphic allorecognition system.
四个为不间断的(AG)n重复序列，一个为(AG)n和(TG)n重复序列。通过一种创新方法，对小群体片段进行最少处理，以作为使用微卫星特异性引物进行PCR的模板。其中四个基因座具有多态性：在九个群体中检测到7 - 8个离散等位基因，杂合度在44% - 80%之间。在基因座811处，等位基因间距和凝胶分辨率最高，因此，对另外十个群体进行了分型，总共检测到九个等位基因，最大等位基因间隔为120个碱基对，杂合群体占53%。微卫星多态性的高水平为研究柄海鞘多态性同种异体识别系统的各个方面提供了一种作为个体标记的新工具。
