Goodison S, Miyazaki J, Ashcroft S J
Nuffield Department of Clinical Biochemistry, University of Oxford, John Radcliffe Hospital, Headington, UK.
Biochem Biophys Res Commun. 1994 Aug 30;203(1):702-10. doi: 10.1006/bbrc.1994.2239.
The mechanism by which glucose stimulates insulin gene expression has been investigated by studying the binding of nuclear proteins to a putative glucose-sensitive element (GSE) in the rat insulin-I gene promoter. Gel retardation assays showed that a specific binding activity was present in four different beta-cell lines. The binding activity was increased by glucose only in those beta-cell lines which were shown to retain glucose-regulated insulin gene transcription. However, a similar binding activity was also shown to be present in an alpha-cell line. The protein factor binding to the GSE was estimated to have a molecular weight of 27kD. This protein may play a pivotal role in glucose-regulated transcription of the insulin gene.
通过研究核蛋白与大鼠胰岛素-I基因启动子中假定的葡萄糖敏感元件（GSE）的结合，对葡萄糖刺激胰岛素基因表达的机制进行了研究。凝胶阻滞试验表明，在四种不同的β细胞系中存在一种特异性结合活性。仅在那些被证明保留葡萄糖调节胰岛素基因转录的β细胞系中，葡萄糖可增加这种结合活性。然而，在一种α细胞系中也显示存在类似的结合活性。与GSE结合的蛋白质因子估计分子量为27kD。这种蛋白质可能在胰岛素基因的葡萄糖调节转录中起关键作用。
