Berrens L, Van Dijk A G
Dev Biol Stand. 1975;29:54-67.
House dust extracts for clinical use must be purified in order to free them from indesirable irritants and pyrogens. A discussion is given of practical and simple methods for the standardized production of purified house dust allergens, which can be quantitated on the basis of relatively simple analytical and physicochemical parameters. Immunoelectrophoretic analysis may help in the controlled production of safe and potent preparations. In contrast, elaborate standardization by quantitative single radial immunodiffusion with anti house dust sera may lead to completely erroneous results.
临床使用的屋尘提取物必须进行纯化，以去除不良刺激物和热原。本文讨论了标准化生产纯化屋尘变应原的实用且简单的方法，这些变应原可根据相对简单的分析和物理化学参数进行定量。免疫电泳分析有助于安全有效地控制制剂的生产。相比之下，使用抗屋尘血清通过定量单向放射免疫扩散进行精细标准化可能会导致完全错误的结果。
