Strejan G H
Dev Biol Stand. 1975;29:79-89.
Ascaris species and their soluble extracts are exceptionally active in inducing atopic sensitization in a very high proportion of exposed individuals and stimulate high levels of reaginic (IgE) antibodies in many experimental animal models. A component responsible for both stimulating reaginic antibodies and eliciting skin reactions in previously sensitized animals was isolated and purified from the crude extract by associating Sephadex gel filtration and polyvinyl chloride block electrophoresis. The allergen (Asc-1) had a molecular weight of 17,000-18,000, had an isoelectric point of 5.0-5.2, contained 8.5% carbohydrate and dissociated in polyacrylamide gel electrophoresis in SDS as a subunit with a molecular weight of 8.400. A single injection of 10 mug protein Asc-1 could stimulate reagin production in rats. Experimental A.suum infections in rats determine reaginic antibody titers ranging from 1:160 after a single infection to 1:4000 after a second infection. The reagins could be detected using Asc-1 as challenge, indicating that the allergen obtained from the crude extract was present in all stages of the parasite life cycle and was directly involved in stimulating reagin production during the migratory phase of the infection. Immunization of rats with Asc-1 in complete Freund's adjuvant completely suppressed the reagin response expected to follow after either one or two infections. Asc-1 did not act as an adjuvant for reagin production nor did an A.suum infection potentiate the reagin response to irrelevant antigens.
蛔虫及其可溶性提取物在极高比例的暴露个体中异常活跃地诱导特应性致敏，并在许多实验动物模型中刺激产生高水平的反应素（IgE）抗体。通过结合葡聚糖凝胶过滤和聚氯乙烯区带电泳，从粗提物中分离并纯化出一种在致敏动物中既能刺激反应素抗体产生又能引发皮肤反应的成分。该变应原（Asc-1）分子量为17,000 - 18,000，等电点为5.0 - 5.2，含8.5%碳水化合物，在SDS聚丙烯酰胺凝胶电泳中解离为分子量8,400的亚基。单次注射10微克蛋白质Asc-1可刺激大鼠产生反应素。大鼠实验性猪蛔虫感染确定的反应素抗体效价范围为单次感染后1:160至二次感染后1:4000。以Asc-1作为激发物可检测到反应素，表明从粗提物中获得的变应原存在于寄生虫生命周期的所有阶段，并在感染的迁移阶段直接参与刺激反应素产生。用Asc-1在完全弗氏佐剂中免疫大鼠可完全抑制一次或两次感染后预期的反应素反应。Asc-1不作为反应素产生的佐剂，猪蛔虫感染也不增强对无关抗原的反应素反应。
