Strzałka K, Gruszecki W I
Department of Plant Physiology and Biochemistry, Jan Zurzycki Institute of Molecular Biology, Jagiellonian University, Kraków, Poland.
Biochim Biophys Acta. 1994 Aug 24;1194(1):138-42. doi: 10.1016/0005-2736(94)90212-7.
The influence of beta-carotene on structural and dynamic properties of model membranes (multilamellar liposomes) prepared of dipalmitoylphosphatidylcholine was investigated. It was found that beta-carotene: (1) decreases order within crystalline state of the membrane; the effect of beta-carotene was more pronounced than in the case of the polar carotenoid, lutein, as revealed by means of spin label EPR; (2) increases penetration, stronger than lutein, of apolar molecules into the membrane as indicated by greater partition coefficient of 5-doxyldecane; (3) increases correlation times tau B tau C stronger than lutein. In all cases the effect of beta-carotene on a membrane was more pronounced at crystalline state than at fluid state. On this basis a hypothesis is proposed that beta-carotene plays a physiological function in the fluidization of chloroplast membranes in a chilling stress to the photosynthetic apparatus.
研究了β-胡萝卜素对由二棕榈酰磷脂酰胆碱制备的模型膜（多层脂质体）的结构和动力学性质的影响。发现β-胡萝卜素：（1）降低膜结晶态内的有序性；如通过自旋标记电子顺磁共振所揭示，β-胡萝卜素的作用比极性类胡萝卜素叶黄素更显著；（2）如5-脱氧癸烷的分配系数更大所示，增加非极性分子进入膜的渗透率，比叶黄素更强；（3）比叶黄素更强烈地增加相关时间τB和τC。在所有情况下，β-胡萝卜素对膜的作用在结晶态比在流体态更显著。在此基础上提出一个假说，即在对光合机构的冷害胁迫中，β-胡萝卜素在叶绿体膜的流化中发挥生理功能。
