Nishiyama S, Okada T, Kobayashi T, Garcia del Saz E, Seguchi H
Department of Otolaryngology, Kochi Medical School, Japan.
Histol Histopathol. 1994 Apr;9(2):205-9.
The effect of endolymphatic hydrops on the Na-K-ATPase activity in the guinea pig stria vascularis was electron microscopically and enzyme cytochemically investigated one year after experimental induction. The morphological observations revealed intercellular dropsy in the basal infoldings of the marginal cells, and shrinkage and disappearance of intermediate cells. Moreover, shrinkage of the marginal cells, especially of the basal infoldings, was occasionally observed. In spite of these morphological alterations, the Na-K-ATPase activity was still detected on the plasma membrane of the basal infoldings of most marginal cells. No remarkable differences were found among the cochlear turns of the specimens examined. However, no reaction product was detected on the basolateral plasma membrane of severely degenerated marginal cells. The present results indicate that the Na-K-ATPase of the plasma membrane of the basal infoldings of the marginal cells plays an important role in the maintenance of the unique ion concentration of the endolymph even in the endolymphatic hydropic condition, and that the Na-K-ATPase activity is attenuated in severely atrophic cells.
实验诱导一年后，通过电子显微镜和酶细胞化学方法研究了内淋巴积水对豚鼠血管纹中钠钾ATP酶活性的影响。形态学观察发现，边缘细胞基底褶内出现细胞间水肿，中间细胞萎缩并消失。此外，偶尔观察到边缘细胞萎缩，尤其是基底褶的萎缩。尽管有这些形态学改变，但在大多数边缘细胞基底褶的质膜上仍可检测到钠钾ATP酶活性。在所检查的标本的耳蜗各圈之间未发现明显差异。然而，在严重退化的边缘细胞的基底外侧质膜上未检测到反应产物。目前的结果表明，即使在内淋巴积水状态下，边缘细胞基底褶质膜上的钠钾ATP酶在内淋巴独特离子浓度的维持中也起着重要作用，并且在严重萎缩的细胞中钠钾ATP酶活性减弱。
