Coto-Montes A, García-Fernández J M, del Brío M A, Riera P
Department of Morphology and Cell Biology, School of Medicine, University of Oviedo, Spain.
Histol Histopathol. 1994 Apr;9(2):233-41.
The study was carried out on the distribution of neurons and fibres which contain the Corticotropin releasing factor-like (CRF) immunoreactivity in the encephalon of two species of teleosts, Gambusia affinis and Salmo trutta. The peroxidase-antiperoxidase (PAP) immunocytochemical technique was employed. The present study has shown differences between both species. In Gambusia affinis, positive neurons were observed in the area ventralis telencephali pars lateralis (VLT), in the nucleus praeopticus (NPO) and in the nucleus lateralis tuberis (NLT). The immunoreactive fibres were in the area ventralis telencephali, in the preoptic hypophyseal tract and in the hypophysis. In Salmo trutta the immunoreactive cells were seen in the pars magnocellularis and pars parvocellularis of the NPO and in cerebrospinal fluid (CSF) contacting neurons of the NLT. The pattern of distribution of immunoreactive fibres in Salmo trutta was different from that in Gambusia affinis. In addition to the distribution of perikarya in Gambusia affinis (in the VLT and in the preoptic hypophyseal tract), fibres were also observed in the tubero-hypophyseal tract and in the posterior hypothalamus. The hypophysis of Salmo trutta also presents an extensive labelling. The interspecific differences shown in the present study should be due to the different degree of evolution in the two species studied and to other causes, such as environmental ones.
本研究针对食蚊鱼（Gambusia affinis）和褐鳟（Salmo trutta）这两种硬骨鱼脑内含有促肾上腺皮质激素释放因子样（CRF）免疫反应性的神经元和纤维分布展开。采用了过氧化物酶抗过氧化物酶（PAP）免疫细胞化学技术。本研究显示了两种鱼之间的差异。在食蚊鱼中，在端脑腹侧部外侧区（VLT）、视前核（NPO）和外侧结节核（NLT）观察到阳性神经元。免疫反应性纤维见于端脑腹侧区、视前垂体束和垂体。在褐鳟中，免疫反应性细胞见于视前核的大细胞部和小细胞部以及外侧结节核的脑脊液（CSF）接触神经元。褐鳟中免疫反应性纤维的分布模式与食蚊鱼不同。除了食蚊鱼中核周体的分布（在VLT和视前垂体束中）外，在结节垂体束和下丘脑后部也观察到纤维。褐鳟的垂体也呈现广泛的标记。本研究中显示的种间差异应归因于所研究的两种鱼不同的进化程度以及其他原因，如环境因素。
