大鼠主要组织相容性复合体III类区域的脉冲场凝胶电泳分析显示出广泛的种间保守性。

Pulsed field gel electrophoretic analysis of the rat major histocompatibility complex class III region shows extensive inter-species conservation.

作者信息

Lund T, Carter C, Campbell R D

机构信息

Department of Immunology and Molecular Pathology, University College London Medical School, UK.

出版信息

Mamm Genome. 1994 May;5(5):282-7. doi: 10.1007/BF00389542.

DOI:10.1007/BF00389542

PMID:8075501

Abstract

Using pulsed field gel electrophoresis (PFGE), we have examined the rat major histocompatibility complex (MHC) for the presence of a number of new class III region genes recently identified in the human MHC. We find homologous genes to the human G1, G2, G4, G7a, G9, G9a, G10, G13, G15, and G18 genes, but not the G8 gene in the rat genome, and show that these are linked to the rat TNF-alpha and C4/Slp loci. A long-range restriction map has been constructed on the basis of a PFGE analysis which demonstrates extensive co-linearity in the positions of the homologous sequences in the region between the C4/Slp and TNF loci in the rat MHC when compared with that of the human MHC class III region.

摘要

我们使用脉冲场凝胶电泳（PFGE）技术，检测了大鼠主要组织相容性复合体（MHC），以寻找人类MHC中最近发现的一些新的Ⅲ类区域基因。我们在大鼠基因组中发现了与人类G1、G2、G4、G7a、G9、G9a、G10、G13、G15和G18基因同源的基因，但未发现G8基因，并表明这些基因与大鼠肿瘤坏死因子-α（TNF-α）和C4/Slp基因座相连。基于PFGE分析构建了一个长距离限制酶切图谱，该图谱表明，与人类MHCⅢ类区域相比，大鼠MHC中C4/Slp和TNF基因座之间区域的同源序列位置具有广泛的共线性。