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通过毛细管区带电泳和激光解吸/电离质谱监测对五步蛇蛇毒中的蛋白质进行分离和鉴定。

Separation and identification of proteins obtained from Agkistrodon acutus snake venom by capillary zone electrophoresis and laser desorption/ionization mass monitoring.

作者信息

Zhang Y, Chen N, Wang L, Yang B, Qian X, Ma L

机构信息

National Chromatographic R. and A. Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences.

出版信息

Biomed Chromatogr. 1994 May-Jun;8(3):148-50. doi: 10.1002/bmc.1130080312.

DOI:10.1002/bmc.1130080312
PMID:8075526
Abstract

Fractions of seven protein principles with fibrinolytic or thrombin-like activities obtained from Agkistrodon acutus snake venom purified by two steps of normal pressure chromatography were separated further by capillary zone electrophoresis (CZE). Mass determination for these fractions were achieved by performing laser desorption/ionization mass monitoring (LDIM). The comparative study between CZE and LDIM on the separation of these fractions was made.

摘要

通过两步常压色谱法纯化的五步蛇蛇毒中获得的具有纤溶或类凝血酶活性的七种蛋白质成分,进一步采用毛细管区带电泳(CZE)进行分离。通过激光解吸/电离质谱监测(LDIM)对这些成分进行质量测定。对CZE和LDIM在分离这些成分方面进行了比较研究。

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