Orfao A, Ciudad J, Lopez-Berges M C, Lopez A, Vidriales B, Caballero M D, Valverde B, Gonzalez M, San Miguel J F
Servicio General de Citometria, Universidad de Salamanca, Spain.
Leuk Lymphoma. 1994;13 Suppl 1:87-90. doi: 10.3109/10428199409052682.
In the present study the usefulness of a method combining multiple staining direct immunofluorescence technique together with flow cytometry in order to predict relapse in ALL is analyzed in a group of 47 patients (11 T-ALL and 36 B-ALL). Results show that this method can be applied to at least two-thirds of all ALL patients being specially useful for the T-ALL cases (100% vs 56%) as this corresponding to the incidence of "aberrant" phenotypes. The detection of an increase in the percentage of bone marrow cells displaying "aberrant" phenotypes in two consecutive samples from the same patient is of great help on predicting relapse (sensitivity of 92% and specificity of 75%).
在本研究中，对47例患者（11例T淋巴细胞白血病和36例B淋巴细胞白血病）组成的队列分析了一种将多重染色直接免疫荧光技术与流式细胞术相结合的方法在预测急性淋巴细胞白血病复发方面的实用性。结果显示，该方法可应用于至少三分之二的急性淋巴细胞白血病患者，对T淋巴细胞白血病病例特别有用（100%对56%），因为这与“异常”表型的发生率相对应。在同一患者的两个连续样本中检测到显示“异常”表型的骨髓细胞百分比增加，对预测复发有很大帮助（敏感性为92%，特异性为75%）。
