Gillardon F, Beck H, Uhlmann E, Herdegen T, Sandkühler J, Peyman A, Zimmermann M
II. Physiologisches Institut, Universität Heidelberg, Germany.
Eur J Neurosci. 1994 May 1;6(5):880-4. doi: 10.1111/j.1460-9568.1994.tb00999.x.
Peripheral noxious stimulation leads to a rapid and transient expression of c-fos, c-jun and other immediate-early genes (IEGs) in the spinal cord. However, the role of IEG encoded transcription factors in plasticity of spinal neurons remains speculative. In the present study we have shown that superfusion of rat spinal cord with antisense oligodeoxynucleotides complementary to c-fos mRNA suppresses heat-induced c-Fos protein expression without affecting other members of the Fos and Jun family, thus providing a technique to determine the function of IEGs in vivo.
外周伤害性刺激可导致脊髓中c-fos、c-jun和其他即刻早期基因（IEGs）快速短暂表达。然而，IEG编码的转录因子在脊髓神经元可塑性中的作用仍具有推测性。在本研究中，我们发现用与c-fos mRNA互补的反义寡脱氧核苷酸灌注大鼠脊髓，可抑制热诱导的c-Fos蛋白表达，而不影响Fos和Jun家族的其他成员，从而提供了一种在体内确定IEGs功能的技术。
