Gajadhar A A, Tessaro S V, Yates W D
Health of Animals Laboratory, Agriculture Canada, Saskatoon, Saskatchewan.
Can Vet J. 1994 Jul;35(7):433-7.
A modified Baermann assay was used to recover dorsal-spined, first stage larvae of Elaphostrongylus cervi from feces and lungs of red deer (Cervus elaphus elaphus) from three of four herds imported from New Zealand into Canadian quarantine facilities. Tests done on a series of fecal collections showed that larval output from infected red deer was low and sporadic, casting doubt on the efficacy of the Baermann assay to detect all infected individuals in the herds. The animals had passed repeated preembarkation Baermann tests for E. cervi in New Zealand. Seven larvae recovered from these red deer were used to establish a patent infection in a naive red deer. The prepatent period was 206 days and larval shedding was intermittent. Elaphostrongylus cervi is a foreign animal parasite in continental North America, which could become irrevocably established if it were introduced. The data reported indicates that there is currently no reliable method for the detection of E. cervi infection.
采用改良的贝尔曼氏法，从从新西兰进口到加拿大检疫设施的四个鹿群中的三个鹿群的马鹿（Cervus elaphus elaphus）粪便和肺中回收鹿狂蝇的背刺第一期幼虫。对一系列粪便样本进行的检测表明，受感染马鹿的幼虫排出量很低且不连续，这让人怀疑贝尔曼氏法检测鹿群中所有受感染个体的有效性。这些动物在新西兰登船前曾多次通过针对鹿狂蝇的贝尔曼氏检测。从这些马鹿中回收的七条幼虫用于在一只未感染的马鹿中建立显性感染。潜伏期为206天，幼虫排出是间歇性的。鹿狂蝇是北美大陆的一种外来动物寄生虫，如果被引入，可能会不可逆转地定殖。报告的数据表明，目前尚无检测鹿狂蝇感染的可靠方法。
