Castresana J S, Barrios C, Gómez L, Kreicbergs A
Department of Tumor Pathology, Karolinska Hospital, Stockholm, Sweden.
Cancer Genet Cytogenet. 1994 Aug;76(1):47-9. doi: 10.1016/0165-4608(94)90070-1.
Oncogenes and tumor suppressor genes coparticipate in sarcoma tumorigenesis. We tested 43 sarcomas for c-myc amplification by Southern blot and molecular hybridization techniques and TP53 mutations by the polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) technique and direct sequencing of the PCR products. We found eight tumors with c-myc amplification and five different tumors with TP53 mutations but no tumors harbor both c-myc and TP53 alterations. We suggest that c-myc amplification and TP53 mutations do not seem to coparticipate in the tumorigenesis of sarcomas.
癌基因和肿瘤抑制基因共同参与肉瘤的肿瘤发生过程。我们采用Southern印迹法和分子杂交技术检测了43例肉瘤的c-myc基因扩增情况，并采用聚合酶链反应-单链构象多态性（PCR-SSCP）技术及PCR产物直接测序法检测了TP53基因突变情况。我们发现8例肿瘤存在c-myc基因扩增，5例不同的肿瘤存在TP53基因突变，但没有肿瘤同时存在c-myc和TP53改变。我们认为c-myc基因扩增和TP53基因突变似乎并不共同参与肉瘤的肿瘤发生过程。
