Dees C, Travis C
Molecular Toxicology Group, Oak Ridge National Laboratory, TN 37831-6109.
Cancer Lett. 1994 Sep 15;84(2):117-23. doi: 10.1016/0304-3835(94)90365-4.
Phorbol 12-myristate, 13-acetate (PMA) is a known protein kinase C activator (PKC); benzene, chloroform, and toluene have also been reported to be PKC activators. We examined the effects of these three solvents on the phosphorylation of p53 in treated cells. Hyperphosphorylated p53 was found when p53 was immunoprecipitated from rat liver epithelial cell extracts treated with any of the solvents or PMA. The solvents also resulted in hyper-phosphorylation of human p53 produced by transfection of Saos-2 cells with a eucaryotic expression vector. Increased phosphorylation of p53 induced by the solvents was also observed through in vitro assays. Hyperphosphorylation of p53 may be involved in tumor promotion by benzene, toluene and chloroform.
佛波醇12 -肉豆蔻酸酯13 -乙酸酯（PMA）是一种已知的蛋白激酶C激活剂（PKC）；据报道，苯、氯仿和甲苯也是PKC激活剂。我们研究了这三种溶剂对经处理细胞中p53磷酸化的影响。当从用任何一种溶剂或PMA处理的大鼠肝上皮细胞提取物中免疫沉淀p53时，发现了过度磷酸化的p53。这些溶剂还导致用真核表达载体转染Saos - 2细胞产生的人p53发生过度磷酸化。通过体外试验也观察到了溶剂诱导的p53磷酸化增加。p53的过度磷酸化可能与苯、甲苯和氯仿的促肿瘤作用有关。
