Field J K, Zoumpourlis V, Spandidos D A, Jones A S
Department of Clinical Dental Sciences, University of Liverpool.
Cancer Detect Prev. 1994;18(3):197-208.
53 expression was assessed in 93 head and neck squamous cell carcinoma patients using CM-1, DO-7, and DO-1 antibodies. Sixty eight percent was found to have positive nuclear staining. The frequency of p53 mutations were investigated in 13 patients using single-stranded conformational polymorphism (SSCP) analysis of DNA fragments that had been amplified by the polymerase chain reaction (PCR). P53 gene mutations were analyzed by SSCP in exons 4 to 7 of 13 patients, and nine were found to have mutations in exon 4; two of these patients also had a mutation in exon 5. In the group of 93 patients, p53 overexpression was found to correlate with the patients' history of heavy smoking (p < 0.01). We also analyzed the drinking and smoking history of 71 of these patients by logistic regression analysis and found that heavy smoking correlates with p53 overexpression (p < 0.05), but heavy drinking was not found to be significant. However when both smoking and drinking histories were assessed together, a correlation was found (p < 0.05). Future work may indicate that specific p53 mutations are associated with patients who have a history of heavy drinking and smoking.
使用CM - 1、DO - 7和DO - 1抗体对93例头颈部鳞状细胞癌患者的53表达进行了评估。发现68%的患者有阳性核染色。使用聚合酶链反应（PCR）扩增的DNA片段的单链构象多态性（SSCP）分析，对13例患者的p53突变频率进行了研究。通过SSCP对13例患者的外显子4至7进行p53基因突变分析，发现9例患者外显子4有突变；其中2例患者外显子5也有突变。在93例患者组中，发现p53过表达与患者的重度吸烟史相关（p < 0.01）。我们还通过逻辑回归分析对其中71例患者的饮酒和吸烟史进行了分析，发现重度吸烟与p53过表达相关（p < 0.05），但未发现重度饮酒有显著相关性。然而，当同时评估吸烟和饮酒史时，发现存在相关性（p < 0.05）。未来的研究可能表明，特定的p53突变与有重度饮酒和吸烟史的患者有关。
