Shulman S, Orsini F R
J Immunol. 1975 Aug;115(2):549-55.
Rabbit vesicular fluid (RVF) was shown to contain only one major electrophoretic component. This component, isolated by starch block electrophoresis, was found to have a molecular weight, by determination of sedimentation and diffusion coefficients, of approximately 17,300. This major component was also effective in isoimmunization. Another component, possibly a minor one, had similar antigenic properties. The major component, and the "minor" one also, were found to be highly tissue-specific (that is, accessory glands-specific) and also species-specific. Isoimmunization procedures have led to the production of autoantibodies. No delayed hypersensitivity response or lesions of the accessory glands of reproduction resulted. It was concluded that the major reason no lesions were found was that the rabbit was not able to develop a delayed hypersensitivity type of response to the components of RVF. When rabbit vesicular fluid was ejaculated as part of the seminal plasma, its molecules were still able to induce an autoantibody response by isoimmunization techniques. This was the same as the response induced by rabbit vesicular fluid, itself.
兔精囊液（RVF）仅含有一种主要的电泳成分。通过淀粉块电泳分离出的该成分，经沉降和扩散系数测定，其分子量约为17300。这种主要成分在同种免疫中也具有效力。另一种成分，可能是次要成分，具有相似的抗原特性。主要成分以及“次要”成分均具有高度的组织特异性（即附属性腺特异性），并且也具有物种特异性。同种免疫程序已导致自身抗体的产生。未出现迟发型超敏反应或生殖附属性腺的病变。得出的结论是，未发现病变的主要原因是兔子无法对兔精囊液成分产生迟发型超敏反应。当兔精囊液作为精浆的一部分射出时，其分子仍能够通过同种免疫技术诱导自身抗体反应。这与兔精囊液本身诱导的反应相同。
