单克隆丙种球蛋白病的特征分析：一种新方法

Characterization of monoclonal gammopathies, a new approach.

作者信息

Nerenberg S T, Mallin J

出版信息

J Lab Clin Med. 1975 Aug;86(2):266-73.

Abstract

A new approach to the characterization (typing) of L- and H-chains of monoclonal immunoglobulins and Bence-Jones protein utilizing elution of a monoclonal immunoglobulin in conjunction with solid-support electrophoresis and the reverse Mancini procedure of radial immunodiffusion on Mylar-backed cellulose acetate membranes is described. A number of benefits are derived, particularly the use of only a fraction (1/200) of the antisera used with agar immunoelectrophoresis. The importance of "standardizing" commercial antisera (and a method for carrying this out) with the techniques described is emphasized.

摘要

本文描述了一种利用单克隆免疫球蛋白洗脱结合固相支持电泳以及在聚酯薄膜支持的醋酸纤维素膜上进行放射免疫扩散的反向曼西尼程序，对单克隆免疫球蛋白和本斯-琼斯蛋白的轻链和重链进行表征（分型）的新方法。该方法有诸多优点，尤其是仅使用琼脂免疫电泳所用抗血清量的一小部分（1/200）。文中强调了使用所述技术对商业抗血清进行“标准化”（以及实施此操作的方法）的重要性。