Ottaviani E, Franchini A, Caselgrandi E, Cossarizza A, Franceschi C
FEBS Lett. 1994 Aug 29;351(1):19-21. doi: 10.1016/0014-5793(94)00802-7.
The addition of corticotropin-releasing factor (CRF) to molluscan hemocytes induces the release of biogenic amines (norepinephrine, epinephrine, dopamine), a phenomenon we have considered as an ancestral type of stress response [(1992) Gen. Comp. Endocrinol. 87, 354-360]. A similar but less significant response was observed following the addition of interleukin-2 (IL-2). Pre-incubation of hemocytes with IL-2 or anti-IL-2 monoclonal antibody significantly reduced or completely eliminated the CRF-induced release of biogenic amines. Further direct evidence of competition between CRF and IL-2 was revealed by immunocytochemical and cytofluorimetric analysis. The data are compatible with the presence of a unique (ancestral?) receptor on molluscan hemocytes, capable of binding both CRF and IL-2, two key molecules of the neuroendocrine and immune system, respectively.
向软体动物血细胞中添加促肾上腺皮质激素释放因子（CRF）会诱导生物胺（去甲肾上腺素、肾上腺素、多巴胺）的释放，我们将这一现象视为一种原始类型的应激反应[（1992年）《普通比较内分泌学》87卷，354 - 360页]。添加白细胞介素-2（IL-2）后观察到类似但不太显著的反应。用IL-2或抗IL-2单克隆抗体对血细胞进行预孵育可显著降低或完全消除CRF诱导的生物胺释放。免疫细胞化学和细胞荧光分析揭示了CRF与IL-2之间竞争的进一步直接证据。这些数据与软体动物血细胞上存在一种独特的（原始的？）受体相符，该受体能够结合CRF和IL-2，它们分别是神经内分泌系统和免疫系统的两个关键分子。
