蛋白激酶C在胆碱能刺激泪腺蛋白质分泌中的作用。
Role of protein kinase C in cholinergic stimulation of lacrimal gland protein secretion.
作者信息
Zoukhri D, Hodges R R, Dicker D M, Dartt D A
机构信息
Schepens Eye Research Institute, Boston, MA 02114.
出版信息
FEBS Lett. 1994 Aug 29;351(1):67-72. doi: 10.1016/0014-5793(94)00824-8.
Abstract
The purpose of this study was to determine the role of protein kinase C (PKC) isozymes in carbachol-induced protein secretion in the lacrimal gland. Three isoforms of PKC are present in rat lacrimal gland; PKC-alpha, -delta and -epsilon. Carbachol translocated PKC-epsilon during 5 s incubation. Pretreatment with PdBu for 0 to 4 h down-regulated PKC-alpha by 31% at 20 min, PKC-epsilon by 36% at 2 h, and PKC-delta by 37% at 4 h. A 2 h phorbol ester treatment inhibited carbachol-induced secretion completely at 1 min and partially at 5, and 20 min, but did not alter the carbachol-induced increase in the intracellular [Ca2+]. We conclude that PKC-alpha and -epsilon, but not PKC-delta, are implicated in cholinergic agonist-induced protein secretion in rat lacrimal gland.
摘要
本研究的目的是确定蛋白激酶C（PKC）同工酶在卡巴胆碱诱导的泪腺蛋白质分泌中的作用。大鼠泪腺中存在三种PKC同工型；PKC-α、-δ和-ε。在孵育5秒期间，卡巴胆碱使PKC-ε易位。用佛波酯（PdBu）预处理0至4小时，在20分钟时使PKC-α下调31%，在2小时时使PKC-ε下调36%，在4小时时使PKC-δ下调37%。佛波酯处理2小时在1分钟时完全抑制了卡巴胆碱诱导的分泌，在5分钟和20分钟时部分抑制，但未改变卡巴胆碱诱导的细胞内[Ca2+]升高。我们得出结论，PKC-α和-ε而非PKC-δ参与了大鼠泪腺中胆碱能激动剂诱导的蛋白质分泌。
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